Description
Description: This assay employs a two-site sandwich ELISA to quantitate YY1AP1 in samples. An antibody specific for YY1AP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YY1AP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YY1AP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YY1AP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YY1AP1 contains the partial promoter and untranslated first exon of ASH1L and coding exons 13 to 21 of GON4L. The 3-prime region of YY1AP1 originated from a long terminal repeat of an endogenous retrovirus, known as the MER51A repeat, that was inserted into exon 21 of GON4L after the duplication.
The deduced YY1AP1 protein contains 750 amino acids, 748 of which correspond to amino acids 590 to 1,337 of the GON4L protein. The last 2 C-terminal residues of YY1AP1 come from the long terminal repeat of MER51A. RT-PCR detected ubiquitous expression of YY1AP1, with highest levels in testis and skeletal muscle. The deduced 467-amino acid protein has a calculated molecular mass of 50.65 kD. The deduced 467-amino acid protein has a calculated molecular mass of 50.65 kD